Figure 4. Pulse/chase analysis of DProPHM-335s and PAM-5. Quadruplicate wells of both cell lines were incubated with [ 35 S]Met/Cys for 15 min and harvested immediately (P15) or chased for 30 min, 120 min, or 240 min before media (Mdm) were harvested and cell extracts prepared (Ch). Samples were analyzed as described in Figure 3.
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