Figure 6. Kinetic analysis of DProPHMs mutants. Aliquots of media prepared as
described in Figure 2 were assayed in the presence of varying concentrations of
a-N -acetyl-Tyr-Val-Gly under standard conditions. Data were plotted in the
Eadie-Hofstee format (Segel, 1975); V , pmol mL -1 h -1 ; V /S , pmol mL -1 h
-1 (mM substrate) -1.
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