Figure 6. Kinetic analysis of DProPHMs mutants. Aliquots of media prepared as described in Figure 2 were assayed in the presence of varying concentrations of a-N -acetyl-Tyr-Val-Gly under standard conditions. Data were plotted in the Eadie-Hofstee format (Segel, 1975); V , pmol mL -1 h -1 ; V /S , pmol mL -1 h -1 (mM substrate) -1.
Back to PHM paper